isms.au.dk

isms.au.dk

Data analysis toolkit for single-molecule FRET TIRF-microscopy. ISMS reads 120 different raw image and movie formats. Determation of correction factors. Time-trace dynamics analysis by Hidden-Markov Modelling (HMM). Gaussian molecule point-spread function (PSF) analysis. Tethered Fluorophore Motion (TFM) analysis. Comments on content: . Revised 30.11.2016. Single-Molecule Fret Microscopy Software. Gustav Wieds Vej 14. E-mail: inano@inano.au.dk. Tel: 45 8715 0000. Fax: 45 8715 0201.

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Data analysis toolkit for single-molecule FRET TIRF-microscopy. ISMS reads 120 different raw image and movie formats. Determation of correction factors. Time-trace dynamics analysis by Hidden-Markov Modelling (HMM). Gaussian molecule point-spread function (PSF) analysis. Tethered Fluorophore Motion (TFM) analysis. Comments on content: . Revised 30.11.2016. Single-Molecule Fret Microscopy Software. Gustav Wieds Vej 14. E-mail: inano@inano.au.dk. Tel: 45 8715 0000. Fax: 45 8715 0201.

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5 study portals

6 nanoscience

7 phd portal

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9 interdisciplinary nanoscience center

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isms.au.dk | isms.au.dk Reviews

https://isms.au.dk

Data analysis toolkit for single-molecule FRET TIRF-microscopy. ISMS reads 120 different raw image and movie formats. Determation of correction factors. Time-trace dynamics analysis by Hidden-Markov Modelling (HMM). Gaussian molecule point-spread function (PSF) analysis. Tethered Fluorophore Motion (TFM) analysis. Comments on content: . Revised 30.11.2016. Single-Molecule Fret Microscopy Software. Gustav Wieds Vej 14. E-mail: inano@inano.au.dk. Tel: 45 8715 0000. Fax: 45 8715 0201.

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isms.au.dk

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Troubleshooting

http://isms.au.dk/troubleshooting

Troubleshooting Problems with iSMS. If you are having problems running the software follow these steps carefully:. Follow the installation instructions. Make sure the requirements listed here. See this page for common issues when installing the software. Check if your problem is covered by the bug fix list below. If none of the above worked out please write us an email. Describing you problem or fill in a bug report. 201507.01 - v1.04: Cannot open file. Error in = iSMS.m at line 95. Download this zip file.

2

Getting started

http://isms.au.dk/getstarted

Installing and Running the Software. Quick Guide 1: FRET-Pairs, Traces and Histograms. Quick Guide 2: Correction Factors. Quick Guide 3: Transitional Dynamics. Quick Guide 4: Automate Analysis. Quick Guide 5: Open and Explore Sessions. Installing and Running the Software. Quick Guide 1: FRET-Pairs, Traces and Histograms. Quick Guide 2: Correction Factors. Quick Guide 3: Transitional Dynamics. Quick Guide 4: Automate Analysis. Quick Guide 5: Open and Explore Sessions. Comments on content: .

3

Download iSMS

http://isms.au.dk/download

Version 2.01 - 2016.20.09. Download iSMS for MATLAB (includes source code). If you need other versions of iSMS, please contact us. Updates in version 2.01. Inclusion of background estimator toolkits described in: Preus, S., Hildebrand, L L., Birkedal V., Optimal Background Estimators in Single-Molecule FRET Microscopy, Biophysical Journal (2016), 111, 6, 1278-1286. Small bugs were fixed in this version. Version 1.06 - 2016.06.07. Download iSMS for MATLAB (includes source code). Download test data below.

4

Frequently Asked Questions

http://isms.au.dk/faq

Q I accidentally deleted a molecule. Can I get it back? Deleted molecules are saved in the recycle bin. To get back a deleted molecule, see this documentation page. Q I have a sample with very high FRET efficiency and no donor signal. How do I detect FRET-pairs? Q I don't know what my camera background is. Which camera background settings should I use then? Use the pixel inspection tool in iSMS. Q How do I automatically align the emission channels? Select single-color excitation scheme. Single-Molecule F...

5

Documentation for iSMS

http://isms.au.dk/documentation

Exploring raw image data. Export Data and Figures. Documentation pages for iSMS. Installing and Running the Software. Quick Guide 1: FRET-Pairs, Traces and Histograms. Quick Guide 2: Correction Factors. Quick Guide 3: Transitional Dynamics. Quick Guide 4: Automate Analysis. Quick Guide 5: Open and Explore Sessions. Exploring raw image data. Export Data and Figures. Comments on content: . Revised 03.03.2017. Single-Molecule Fret Microscopy Software. Gustav Wieds Vej 14. E-mail: inano@inano.au.dk.

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fluortools.com

News and updates - The fluorescence laboratory.

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ImageA - Image Analysis Software. A e - UV-Vis-IR Spectral Software. DecayFit - Fluorescence Decay Analysis. AniFit - Anisotropy Decay Analysis. FRETmatrix - Simulation of FRET. A e Documentation Pages. How to cite software from FluorTools.com. Troubleshooting: Common installation issues. Wish for a new simulation and data analysis software. Step-by-step tutorial of an abstract scientific illustration. PhD thesis LaTeX template. Easy to use, functional and efficient.". Posted 30 Jun 2015, 16:44. Søren Pr...

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isms.au.dk

Data analysis toolkit for single-molecule FRET TIRF-microscopy. ISMS reads 120 different raw image and movie formats. Determation of correction factors. Time-trace dynamics analysis by Hidden-Markov Modelling (HMM). Gaussian molecule point-spread function (PSF) analysis. Tethered Fluorophore Motion (TFM) analysis. Comments on content: . Revised 30.11.2016. Single-Molecule Fret Microscopy Software. Gustav Wieds Vej 14. E-mail: inano@inano.au.dk. Tel: 45 8715 0000. Fax: 45 8715 0201.

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