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The AdZ adenovirus cloning system | AdZ.cf.ac.uk

Skip to main content. The AdZ adenovirus cloning system. AdZ: A recombinant Adenovirus (Ad) vector with Zero cloning steps. A complete Ad5 vector is carried on a Bacterial Artificial Chromosome (BAC). The DNA insert is transformed directly into cells carrying the AdZ BAC. The insert can be:. Synthetic oligonucleotides (e.g. encoding shRNAs). Conventional plasmid clone (e.g. from an expression library). Recombineering is performed: the transgene replaces dual selectable marker. Website by Richard Stanton.

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The AdZ adenovirus cloning system | AdZ.cf.ac.uk | adz.cf.ac.uk Reviews

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Skip to main content. The AdZ adenovirus cloning system. AdZ: A recombinant Adenovirus (Ad) vector with Zero cloning steps. A complete Ad5 vector is carried on a Bacterial Artificial Chromosome (BAC). The DNA insert is transformed directly into cells carrying the AdZ BAC. The insert can be:. Synthetic oligonucleotides (e.g. encoding shRNAs). Conventional plasmid clone (e.g. from an expression library). Recombineering is performed: the transgene replaces dual selectable marker. Website by Richard Stanton.

<META> KEYWORDS

1 adzcfac uk

2 navigation

3 protocols

4 vector maps

5 getting the reagents

6 frequently asked questions

7 you are here

8 transgene insertion

9 pcr product

10 synthesized gene

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The AdZ adenovirus cloning system | AdZ.cf.ac.uk | adz.cf.ac.uk Reviews

https://adz.cf.ac.uk

Skip to main content. The AdZ adenovirus cloning system. AdZ: A recombinant Adenovirus (Ad) vector with Zero cloning steps. A complete Ad5 vector is carried on a Bacterial Artificial Chromosome (BAC). The DNA insert is transformed directly into cells carrying the AdZ BAC. The insert can be:. Synthetic oligonucleotides (e.g. encoding shRNAs). Conventional plasmid clone (e.g. from an expression library). Recombineering is performed: the transgene replaces dual selectable marker. Website by Richard Stanton.

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adz.cf.ac.uk

1

Vector Maps | AdZ.cf.ac.uk

http://adz.cf.ac.uk/content/vector-maps

Skip to main content. PAdZ5-CV5 - this is the entire sequence, including all the Ad backbone. The following maps are all just the expression cassettes - the remaining sequence is identical to pAdZ5-CV5 above. PAdZ5-CV5 - for adding C terminal V5 tags. PAdZ5-NV5 for adding N terminal V5 tags. PAdZ5-NGFP for adding N terminal GFP tags. PAdZ5-CGFP for adding C terminal GFP tags. PAdZ5-CmCherry for adding C terminal mCherry tags. PAdZ5-mIR155 for cloning shRNAs. Website by Richard Stanton.

2

Protocols | AdZ.cf.ac.uk

http://adz.cf.ac.uk/content/protocols

Skip to main content. Cloning genes into the AdZ vectors and making virus. To download the protocol (pdf format) for inserting genes with any tag into the AdZ vectors. Note that the vector numbers are different to those in the original publication - the original work used vectors in which the genome was derived from the AdEasy vectors. The new vectors contain a newly derived and sequenced Ad5 genome, but the expression cassettes etc remain identical to those in the original paper.

3

Getting the reagents | AdZ.cf.ac.uk

http://adz.cf.ac.uk/content/getting-reagents

Skip to main content. If you'd like to obtain the AdZ reagents, we have an MTA in place. We distribute the reagents on a not-for-profit basis, please contact. Us with your details. Website by Richard Stanton.

4

FAQ | AdZ.cf.ac.uk

http://adz.cf.ac.uk/content/faq

Skip to main content. Can I make the bacteria competent, then freeze them for later use? According to others, freezing the bacteria results in 10-fold drop in recombination efficiency, thus we usually use fresh bacteria. For small inserts, that may be acceptable. If you want to try it then instead of washing with water, wash with 10% glycerol then freeze after the washing steps. What other selection systems are compatible with the AdZ system? Selection. We've also successfully used genebridges.

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biotechniques.com

BioTechniques - Re-engineering adenovirus vector systems to enable high-throughput analyses of gene function

http://www.biotechniques.com/BiotechniquesJournal/2008/December/Re-engineering-adenovirus-vector-systems-to-enable-high-throughput-analyses-of-gene-function/biotechniques-45726.html

Forgotten your login details? To BioTechniques free email alert service to receive content updates. Re-engineering adenovirus vector systems to enable high-throughput analyses of gene function. Richard J. Stanton. Brian P. McSharry. And Gavin W.G. Wilkinson. Department of Medical Microbiology, Tenovus Building, Cardiff University, Heath Park, Cardiff, UK. BioTechniques, Vol. 45, No. 6, December 2008, pp. 659–668. And as a delivery platform for RNAi ( 2. The E3 region is nonessential for replication in vi...

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The AdZ adenovirus cloning system | AdZ.cf.ac.uk

Skip to main content. The AdZ adenovirus cloning system. AdZ: A recombinant Adenovirus (Ad) vector with Zero cloning steps. A complete Ad5 vector is carried on a Bacterial Artificial Chromosome (BAC). The DNA insert is transformed directly into cells carrying the AdZ BAC. The insert can be:. Synthetic oligonucleotides (e.g. encoding shRNAs). Conventional plasmid clone (e.g. from an expression library). Recombineering is performed: the transgene replaces dual selectable marker. Website by Richard Stanton.

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