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Cell Culture Protocols for Hunks. Bioinformatics Protocols for Hunks. RNA Protocols for Hunks. Protein Protocols for Hunks. Lipidology Protocols for Hunks. Animal Protocols for Hunks. Mammalian Cell Freezing Media Recipe. Construction of PDMS - Glass Culture Platform. 1 Heat the oven to 65oC. 2 Poly(dimethyl siloxane) (PDMS) will be used in a 10:1 ratio of base to. Curing agent (SYLGARD 184 Silicone Elastomer). 3 In a plastic cup, weigh approximately 30g of the elastomer base. 4 Add 3g of the curing agent.
