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Targeting Vectors | Human Genome Editing Core

http://hgec.uchc.edu/targeting-vectors

Skip to UConn Search. Human Genome Editing Core. Search in http:/ hgec.uchc.edu/. Human Genome Editing Core. Search in http:/ hgec.uchc.edu/. Generation of Targeting Vector. Endo-free DNA prep for targeting. Linearization of targeting vector. Design and ordering of single stranded oligo for targeting vector.

2

CRISPR/Cas9 | Human Genome Editing Core

http://hgec.uchc.edu/crisprcas9

Skip to UConn Search. Human Genome Editing Core. Search in http:/ hgec.uchc.edu/. Human Genome Editing Core. Search in http:/ hgec.uchc.edu/. CRISPR Construction and Sequencing. Each additional CRISPRS made at the same time. CRISPR validation (in-vitro or in-vivo) on exogenous DNA. CRISPR validation on genomic loci.

3

TALENS | Human Genome Editing Core

http://hgec.uchc.edu/talens

Skip to UConn Search. Human Genome Editing Core. Search in http:/ hgec.uchc.edu/. Human Genome Editing Core. Search in http:/ hgec.uchc.edu/. Generation of TALEN and sequencing. Verification of TALEN (In-Vitro or In-Vivo expression on exongenous DNA). Verification of TALEN (In-Vivo expression on genomic locus).

4

Cell Culture Services | Human Genome Editing Core

http://hgec.uchc.edu/cell-culture-services

Skip to UConn Search. Human Genome Editing Core. Search in http:/ hgec.uchc.edu/. Human Genome Editing Core. Search in http:/ hgec.uchc.edu/. Cells are targeted by electroporation and plated to perform selection with antibiotics. Number of clones from any targeting experiment can vary widely so fee structure is based upon a per clone basis. Cell culture of Investigators cells for use in targeting. Genotyping and sequencing of surviving clones (first). Expansion of positive clones. 1400 per vial frozen.

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ipscc.uchc.edu ipscc.uchc.edu

Induced Pluripotent Stem Cell and Chromosome Core › University of Connecticut Health Center

http://ipscc.uchc.edu/index.html

Induced Pluripotent Stem Cell and Chromosome Core. Induced Pluripotent Stem Cell Services. Introducing a New Cross-Campus Core. The iPSCC will provide services, training and products related to pluripotent stem cells including iPS cell derivation and iPS cell vector production at the Cell and Genome Sciences Building in Farmington, Connecticut. More About This Site ›. Farmington, CT 06030. Judy Brown, Ph.D., C.G., M.B. (ASCP). Email: judy.brown@uconn.edu. University of Connecticut Health Center.

stemcellcore.uchc.edu stemcellcore.uchc.edu

UConn-Wesleyan University Stem Cell Core › UConn Health

http://stemcellcore.uchc.edu/index.html

University of Connecticut-Wesleyan University Stem Cell Core. Distribution of hESC/iPSC Lines. Derivation of hiPSC Lines. Quality Control Serivces and Reagents. University of Connecticut-Wesleyan University Stem Cell Core. Initially supported with institutional funds from UConn, and subsequently by the Connecticut State Stem Cell Research Program, we have expanded to incorporate a Genetics and Genomics Division. By collaborating with the Gene Targeting and Transgenics Facility at UConn Health. Training f...

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Home | Human Genome Editing Core

Skip to UConn Search. Human Genome Editing Core. Search in http:/ hgec.uchc.edu/. Human Genome Editing Core. Search in http:/ hgec.uchc.edu/. The core can also create custom targeting vectors to be used in combination with TALEN/CRISPRS to create point mutation KIs, correction of point mutations in disease cell lines, or reporter vectors. Consultation to discuss a potential project is at no charge. Following a consultation, a project outline can be developed and a custom quote made for your particula...

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