agd.path.unimelb.edu.au
Sequencing Reaction Protocol : Forms & Protocols : Applied Genetics Diagnostics
http://agd.path.unimelb.edu.au/forms/sequencing-reaction-protocol.html
Centre for Translational Pathology. Forms, Protocols and Links. Sequencing reactions are performed by PCR using the following conditions:. Make up to a final volume of 20µl. Should not exceed 10µl. For BAC DNA add 4µl of BDv3.1 and 2µl of Dilution Buffer. For GC rich templates add DMSO/glycerol to a final 5% (v/v) concentration. Please note that when determining how much DNA to add to a reaction, the entire plasmid size should be taken into account and not just the insert. Tiffany Cowie, Pathology.
agd.path.unimelb.edu.au
Forms & Protocols : Centre for Translational Pathology
http://agd.path.unimelb.edu.au/forms
Centre for Translational Pathology. Forms, Protocols and Links. Forms, Protocols and Links. Request for Sequencing Reaction Set-up and Electrophoresis. Head of Department, Pathology, MDHS. The University of Melbourne. 84 002 705 224. Provider Number: 00116K : Visa information. Comment on this web site.
agd.path.unimelb.edu.au
Sequencing and Genotyping Facility : Centre for Translational Pathology
http://agd.path.unimelb.edu.au/electrophoresis-only/index.html
Centre for Translational Pathology. Forms, Protocols and Links. Melbourne Translational Genomics Platform. The Melbourne Translational Genomics Platform (MTGP) leverages the genetic and genomic sequencing capabilities of the CTP to make these technologies more readily accessible to researchers, clinicians and students across the precinct. The MTGP offers a range of Massively Parallel Sequencing services; contact Kym for more information. Phone: 61 3 9035 7865. Fax: 61 3 9348 2952. Phone: 61 3 8344 3438.
agd.path.unimelb.edu.au
Genetic Diagnostics : Centre for Translational Pathology
http://agd.path.unimelb.edu.au/genetic-diagnostics
Centre for Translational Pathology. Forms, Protocols and Links. Unit (MDx) of the CTP evolved out of the Applied Genetic Diagnostics laboratory that operated out of the Department for over a decade. We continue to offer a broad range of diagnostic screens for neurodegenerative disease, and now offer a suite of analyses for somatic mutation detection (see below). Phone: 61 3 8344 3438. Fax: 61 3 9348 2952. Email: mdx-enquiries@lists.unimelb.edu.au. APP (amyloid precursor protein). PSEN 1 (presenilin 1).
agd.path.unimelb.edu.au
About Us : Centre for Translational Pathology
http://agd.path.unimelb.edu.au/about
Centre for Translational Pathology. Forms, Protocols and Links. The Head of the Department of Pathology and Director of the CTP. Tiffany Cowie (research manager). Michael Christie (pathology fellow). Kym Pham (platform manager). Arthur Hsu (post-doctoral fellow). Clare Love (research assistant). Renate Marquis-Nicholson (pathology fellow). Is a post-doctoral research fellow and the senior scientist of the Medical Diagnostics Laboratory (MDX). Voula Dimitriadis (Senior Technical Officer). 84 002 705 224.