mitochondrialgenome.org

Tomato mitochondrial genome

Subcellular fractionation and mtDNA extraction. Library construction and standard nomenclature. In house pipeline for sequence analysis. Scotti N., Cardi T. Anda Marechal-Drouard L. (2001) Mitochondrial DNA and RNA isolation from small amounts of Potato tissue. Plant Molecular Biology Reporter 19;67a-67h. Gianniny C., Stoeva P., Cheely A. and Dimaculangan D. (2004) RAPD analysis of mtDNA from tomato flowers freee of nuclear DNA artifacts. Biotechniques 36:772-776.

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Tomato mitochondrial genome | mitochondrialgenome.org Reviews

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Subcellular fractionation and mtDNA extraction. Library construction and standard nomenclature. In house pipeline for sequence analysis. Scotti N., Cardi T. Anda Marechal-Drouard L. (2001) Mitochondrial DNA and RNA isolation from small amounts of Potato tissue. Plant Molecular Biology Reporter 19;67a-67h. Gianniny C., Stoeva P., Cheely A. and Dimaculangan D. (2004) RAPD analysis of mtDNA from tomato flowers freee of nuclear DNA artifacts. Biotechniques 36:772-776.

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1 tools

2 blast

3 downloads

4 bioproject

5 clone request

6 methods

7 backend

8 members only

9 background

10 provisional map

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Tomato mitochondrial genome | mitochondrialgenome.org Reviews

https://mitochondrialgenome.org

Subcellular fractionation and mtDNA extraction. Library construction and standard nomenclature. In house pipeline for sequence analysis. Scotti N., Cardi T. Anda Marechal-Drouard L. (2001) Mitochondrial DNA and RNA isolation from small amounts of Potato tissue. Plant Molecular Biology Reporter 19;67a-67h. Gianniny C., Stoeva P., Cheely A. and Dimaculangan D. (2004) RAPD analysis of mtDNA from tomato flowers freee of nuclear DNA artifacts. Biotechniques 36:772-776.

INTERNAL PAGES

mitochondrialgenome.org

1

BLAST Search for Tomato Mitochondrial Genome Project

http://www.mitochondrialgenome.org/BLAST

Choose program to use and database to search:. Tomato Mitochondrial Genome v1.50 (15/10/10). Tomato Mitochondrial Genome v1.40 (14/06/09). Tomato Mitochondrial Genome v1.30 (07/01/09). Tomato Mitochondrial Genome v1.20 (21/06/08). Potato Mitochondrial Genome (25/03/08). All Plant Mitochondrial Genomes (2010). Enter sequence below in FASTA. Or load it from disk. The query sequence is filtered. For low complexity regions by default. Mask for lookup table only. Query Genetic Codes (blastx only).

2

Tomato mitochondrial genome

http://www.mitochondrialgenome.org/methods.html

Subcellular fractionation and mtDNA extraction. Library construction and standard nomenclature. Purified mtDNA fragments are repaired and ligated into the pMOS-Blue (pMOSBlue Blunt Ended Cloning Kit, Amersham Bioscience) and pZERO-2 vectors (Invitrogen) following the manufacture instructions. Selected clones are classified and stored in freeze media in 96-well plates according the following nomenclature: SlmiNNZZZXY. This code accounts for:. XY: well place in the plate. Base calling by Phred software.

3

Tomato mitochondrial genome: Clone Request Form

http://www.mitochondrialgenome.org/clonerequest.html

Subcellular fractionation and mtDNA extraction. Library construction and standard nomenclature. In house pipeline for sequence analysis. In order to facilitate scientific progress and to serve scientific community with the material generated in this project, the Biotechnology Institute (INTA) distributes individual clones upon request only for research purposes. We do not charge handling, nor material costs, however, delivery costs will be paid by recipients. Requested clones (up to five):.

4

Tomato mitochondrial genome

http://www.mitochondrialgenome.org/downloads

Subcellular fractionation and mtDNA extraction. Library construction and standard nomenclature. In house pipeline for sequence analysis. Solanum lycopersicum mitochondrion Accession: AFYB00000000. SOLYC MT v1.50.zip.

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Tomato mitochondrial genome

Subcellular fractionation and mtDNA extraction. Library construction and standard nomenclature. In house pipeline for sequence analysis. Scotti N., Cardi T. Anda Marechal-Drouard L. (2001) Mitochondrial DNA and RNA isolation from small amounts of Potato tissue. Plant Molecular Biology Reporter 19;67a-67h. Gianniny C., Stoeva P., Cheely A. and Dimaculangan D. (2004) RAPD analysis of mtDNA from tomato flowers freee of nuclear DNA artifacts. Biotechniques 36:772-776.

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