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OMEGA中国总代理—广州飞扬生物工程有限公司

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OMEGA中国总代理—广州飞扬生物工程有限公司 | omegabiotek.com.cn Reviews

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选型指南 · Guide-广州飞扬生物工程有限公司

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DNA and RNA Clean Up. PCR Related / Marker.

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DNA and RNA Clean Up. PCR Related / Marker.

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技术·Technologies-选型指南 · Guide-广州飞扬生物工程有限公司

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DNA and RNA Clean Up. PCR Related / Marker.

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DNA and RNA Clean Up. PCR Related / Marker. Taq plus DNA polymerase. Soil RNA Mini Kit. Total DNA/RNA Isolation Kit. FFPE RNA Isolation Kit. Total RNA Kit I. HP Plant DNA Kit.

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日期 2015/4/27 9:12:47 人气 1115. 抗原修复液: 0.01M的柠檬酸钠缓冲液 58.82g柠檬酸三钠及7.56g柠檬酸溶于200ml纯水,调pH至 6.0,纯水1:100稀释为工作液. 10X PBS: 80g NaCl,2g KCl, 14.4g Na2HPO4 和2.4g KH2PO4 加1 L纯水,调pH至 7.4. 洗涤液: 1 PBS 纯水稀释10 PBS 1 PBST 含0.05% Tween-20 的1 PBS 1 PBST super block,生物素标记的UltraTek Anti-Polyvalent二抗,酶标亲和素UltraTek HRP,DAB. 显色试剂盒 Cat. KT1002a. 05%盐酸-乙醇 0.5 1%盐酸,95.0 95.5%乙醇. 苏木素 苏木精2g溶于250ml无水乙醇,十六水合硫酸铝17.6g 溶与750ml纯水,以上溶液充分混合,加入碘酸钠 0.2g和冰醋酸20ml. 组织固定 烘箱温度65-75 30min或者65 过夜. 封闭 加入适当体积的super block KT1002a Reagent A ,37 温箱孵育5min.

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第二代测序技术的核心思想是边合成边测序,即通过捕捉新合成的末端的标记来确定DNA的序列,现有的技术平台主要包括AppliedBiosy stems SOLID system.

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