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The illustrations and texts originate from the submitted manuscript (Badertscher et al.)

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RibosomeBiogenesis.org - Home | ribosomebiogenesis.org Reviews
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The illustrations and texts originate from the submitted manuscript (Badertscher et al.)
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1 biology
2 screening
3 sirna screening procedure
4 imaging
5 image analysis
6 phenotypic analysis
7 hit classification
8 results
9 coupons
10 reviews
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biology,screening,sirna screening procedure,imaging,image analysis,phenotypic analysis,hit classification,results
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RibosomeBiogenesis.org - Home | ribosomebiogenesis.org Reviews

https://ribosomebiogenesis.org

The illustrations and texts originate from the submitted manuscript (Badertscher et al.)

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1

Results - RibosomeBiogenesis.org

http://www.ribosomebiogenesis.org/results.html

RNAi libraries and plate layout. ETH Zurich, Institute of Biochemistry.

2

Phenotypic analysis - RibosomeBiogenesis.org

http://www.ribosomebiogenesis.org/phenotypic-analysis.html

RNAi libraries and plate layout. Phenotypic classification using supervised machine learning. Classification of cells into predefined groups was performed by supervised machine learning as described ( Horvath et al. 2011. Wild et al. 2010. ETH Zurich, Institute of Biochemistry.

3

RNAi libraries and plate layout - RibosomeBiogenesis.org

http://www.ribosomebiogenesis.org/rnai-libraries-and-plate-layout.html

RNAi libraries and plate layout. RNAi screening libraries and plate layout. For the validation screens, a custom-designed library was purchased from Ambion comprising 3000 siRNAs targeting the top 1000 candidates from the genome-wide screen. These siRNAs were distributed on 15 384-well plates together with the appropriate controls. ETH Zurich, Institute of Biochemistry.

4

Hit classification - RibosomeBiogenesis.org

http://www.ribosomebiogenesis.org/hit-classification.html

RNAi libraries and plate layout. To enable a reliable analysis of the screening data, we defined a cell number cut-off of 50 and 200 cells for the primary and secondary screen, respectively. The major readout of our analysis was the ratio of cells displaying nuclear accumulation of the RPS2-YFP over all reporter and Hoechst-positive interphase cells, a number called score (hit rate)( Wild et al., 2010. ETH Zurich, Institute of Biochemistry.

5

Image analysis - RibosomeBiogenesis.org

http://www.ribosomebiogenesis.org/image-analysis.html

RNAi libraries and plate layout. To identify cells and extract their properties, the CellProfiler program ( Carpenter et al., 2006. ETH Zurich, Institute of Biochemistry.

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RibosomeBiogenesis.org - Home

RNAi libraries and plate layout. The illustrations and texts originate from the submitted manuscript (Badertscher et al.). ETH Zurich, Institute of Biochemistry.

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