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The DECIPHER Project - An Open Source shRNA library RNAi Screening Platform

The objectives of the open source DECIPHER Project are to provide academic labs with free tools to perform and analyze comprehensive RNAi knockdown screens with pooled lentiviral shRNA libraries, and to develop a standardized yet versatile platform for collecting and comparing results from different studies and labs. Open source bi-specific (bispecific) SL DDR shRNA libraries are now available free of charge to academic and non-profit researchers.

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The DECIPHER Project - An Open Source shRNA library RNAi Screening Platform | decipherproject.net Reviews

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The objectives of the open source DECIPHER Project are to provide academic labs with free tools to perform and analyze comprehensive RNAi knockdown screens with pooled lentiviral shRNA libraries, and to develop a standardized yet versatile platform for collecting and comparing results from different studies and labs. Open source bi-specific (bispecific) SL DDR shRNA libraries are now available free of charge to academic and non-profit researchers.

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The DECIPHER Project - An Open Source shRNA library RNAi Screening Platform | decipherproject.net Reviews

https://decipherproject.net

The objectives of the open source DECIPHER Project are to provide academic labs with free tools to perform and analyze comprehensive RNAi knockdown screens with pooled lentiviral shRNA libraries, and to develop a standardized yet versatile platform for collecting and comparing results from different studies and labs. Open source bi-specific (bispecific) SL DDR shRNA libraries are now available free of charge to academic and non-profit researchers.

INTERNAL PAGES

decipherproject.net

1

Current shRNA Library Projects - DECIPHER Project

http://www.decipherproject.net/shrna-libraries

Current Pooled Library Projects. Bi-Specific Synthetic Lethality (SL) DDR shRNA Libraries. Page for more information. DECIPHER Project Pooled shRNA Libraries. For additional information on the DECIPHER shRNA Libraries.

2

DECIPHER Data Analysis Software - DECIPHER Project

http://www.decipherproject.net/software

DECIPHER Data Analysis Software. The following software tools to aid in data analysis and sequences of functional shRNA are available as part of the DECIPHER Project. Please refer to the NIH Data and Resource Sharing Policy. For terms and conditions of use. Barcode Analyzer and Deconvoluter. To receive an activation code granting a single computer license. Download Barcode Deconvoluter software, 64-bit. Compatible only with 64-bit versions of Windows 7, Vista, and XP). Required Module Library (BLIB) files.

3

Support - DECIPHER Project

http://www.decipherproject.net/support

Pooled Lentiviral shRNA Library User Manual. Product Analysis Certificate (PAC), Human Modules 1, 2, and 3 in pRSI12 vector. Product Analysis Certificate (PAC), Mouse Modules 1 and 2 in pRSI12 vector. Barcode Deconvoluter Software User Manual. Complete Gene Annotations, Human. Complete Gene Annotations, Mouse. Complete shRNA and Barcode Sequences for all DECIPHER libraries in pRSI9 or pRSI12. Including pRSI12 Plasmid Library HT Sequencing QC (also used as control). DECIPHER shRNA Expression Vector.

4

Bi-Specific Synthetic Lethal (SL) DDR shRNA Libraries - Project Summary - DECIPHER Project

http://www.decipherproject.net/shRNA-libraries/bi-specific

Bi-Specific Synthetic Lethal (SL) DDR shRNA Libraries – Project Summary. Developed under an NCI-supported synthetic lethality RNAi screening and DDR database contract research project. NCI Topic 290: siRNA Resource for Synthetic Lethal Screening of DNA Repair and Damage Signaling Networks. Development of technology and commercial reagents for combinatorial RNAi screen of 360 DDR genes. Validation of combinatorial RNAi reagents in synthetic lethality screen in 3 cancer cell lines. Under HHSN261201200065C ...

5

Site Map - DECIPHER Project

http://www.decipherproject.net/sitemap

Current shRNA Library Projects. Bi-Specific Synthetic Lethal (SL) DDR shRNA Libraries – Project Summary. Bi-Specific SL DDR shRNA Libraries – Project Details. DECIPHER Data Analysis Software. DECIPHER Mission and Accomplishments. NIH Data and Resource Sharing Policy. Privacy Policy and Terms of Use. Reporter Assay for HT Identification of Functional shRNA. Frequently Asked Questions (FAQs).

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prlog.org

Addgene Helps Distribute Cellecta’s DECIPHER shRNA Libraries to the Academic Community -- Melanie Herscovitch | PRLog

https://www.prlog.org/11495700-addgene-helps-distribute-cellectas-decipher-shrna-libraries-to-the-academic-community.html

Submit Free Press Release. Addgene Helps Distribute Cellecta’s DECIPHER shRNA Libraries to the Academic Community. Addgene connects with the DECIPHER Project to help distribute shRNA Libraries to the scientific community. May 17, 2011. Decipher). shRNAs represent a form of RNAi that can be used to silence genes in a variety of cell types. The DECIPHER lentiviral shRNA libraries are robust tools that researchers can use for functional gene studies and drug discovery initiatives. We would like to make the ...

cellecta.com

Gene Knockdown or Knockout Validation - Cellecta, Inc

https://www.cellecta.com/products-services/cellectas-complete-loss-of-function-screening-services/gene-knockdown-or-knockout-validation

KO / KD Validation. Expression & Reporter. RNAi and shRNA Design. ShRNA and CRISPR Libraries. Webinar: RNAi & CRISPR Screens. User Manuals / PACs. KO / KD Validation. Gene Knockdown or Knockout Validation. Cellecta can help validate target genes by knocking down expression in your cells of interest. Cellecta provides complete services for validation of target genes including qRT-PCR, proliferation assays, reporter assays, and custom stable knockdown or knockout cell line creation. 10 week turnaround time.

cellecta.com

Next-Gen Sequencing and Analysis - Cellecta, Inc

https://www.cellecta.com/products-services/cellecta-pooled-lentiviral-shrna-and-sgrna-libraries/ht-sequencing-and-analysis

KO / KD Validation. Expression & Reporter. RNAi and shRNA Design. ShRNA and CRISPR Libraries. Webinar: RNAi & CRISPR Screens. User Manuals / PACs. Next-Gen Sequencing and Analysis. Cellecta now provides sequencing of samples screened with GeCKO and other libraries. For more information, please inquire. You provide Cellecta with frozen cell pellets or tissue after screening with Cellecta or GeCKO libraries. Cellecta does the rest:. Amplifies barcodes or sgRNA. Performs NGS on the Illumina NextSeq or HiSeq.

cellecta.com

Loss-of-Function Screening Services - Cellecta, Inc

https://www.cellecta.com/products-services/cellectas-complete-loss-of-function-screening-services

KO / KD Validation. Expression & Reporter. RNAi and shRNA Design. ShRNA and CRISPR Libraries. Webinar: RNAi & CRISPR Screens. User Manuals / PACs. KO / KD Validation. Cellecta’s Complete Loss-of-Function Screening Services. Comprehensive loss-of-function screens by experienced scientists using the best shRNA and sgRNA pooled libraries available. Viability and FACS assays based on your experimental needs and in your model cell lines. A Unique and Powerful Approach to Identify Genes. Sequencing of samples ...

cellecta.com

Isogenic Cell Line Engineering - Cellecta, Inc

https://www.cellecta.com/products-services/cell-line-engineering

KO / KD Validation. Expression & Reporter. RNAi and shRNA Design. ShRNA and CRISPR Libraries. Webinar: RNAi & CRISPR Screens. User Manuals / PACs. Custom Isogenic Cell Lines. Isogenic Cell Line Applications. Understanding disease models and gene-drug association. A range of stable clones can easily be generated and then selected for complete knockout or optimum knockdown, expression, or activation, depending on the experimental requirements. An almost unlimited number of stable clones can be produced...

cellecta.com

HT Genetic Screening Products and Services - Cellecta, Inc

https://www.cellecta.com/products-services

KO / KD Validation. Expression & Reporter. RNAi and shRNA Design. ShRNA and CRISPR Libraries. Webinar: RNAi & CRISPR Screens. User Manuals / PACs. HT Genetic Screening Products and Services. To discuss. Much of our technology development begins as collaborations to solve practical research challenges. CRISPR Products and Services. ShRNA Products and Services. HT Genetic Screening Services. Pooled Lentiviral CRISPR, shRNA, and Barcode Libraries. Isogenic Cell Line Engineering. Product & Services.

cellecta.com

Custom shRNA and CRISPR Libraries - Cellecta, Inc

https://www.cellecta.com/products-services/cellecta-pooled-lentiviral-shrna-and-sgrna-libraries/custom-shrna-and-sgrna-libraries

KO / KD Validation. Expression & Reporter. RNAi and shRNA Design. ShRNA and CRISPR Libraries. Webinar: RNAi & CRISPR Screens. User Manuals / PACs. Custom Pooled shRNA and CRISPR Libraries. Construction of shRNA and CRISPR Libraries. A higher percentage of functional shRNAs, designed using validated shRNA sequences and optimized shRNA structures. Flexible array-based oligonucleotide synthesis enables rapid creation of complex libraries expressing any set of shRNAs and sgRNAs to any targets. For broad cove...

cellecta.com

Human Genome-Wide CRISPR sgRNA Library - Cellecta, Inc

https://www.cellecta.com/products-services/cellecta-pooled-lentiviral-shrna-and-sgrna-libraries/human-crispr-genome-wide-sgrna-library

KO / KD Validation. Expression & Reporter. RNAi and shRNA Design. ShRNA and CRISPR Libraries. Webinar: RNAi & CRISPR Screens. User Manuals / PACs. Human Genome-Wide CRISPR sgRNA Library. For Genome-Wide Coverage in Human. 3 Modules, each covering approximately 6,500 genes. Each gene is targeted by up to 8 sgRNA for a total of up to 55,000 sgRNA per module. Targeting a total of over 19,000 protein encoding genes. Compatible with Illumina HiSeq, GAIIx, and NextSeq NGS systems. Download the SnapGene Viewer.

cellecta.com

Human Genome-Wide shRNA Library - Cellecta, Inc

https://www.cellecta.com/products-services/cellecta-pooled-lentiviral-shrna-and-sgrna-libraries/human-genome-wide-shrna-library-2

KO / KD Validation. Expression & Reporter. RNAi and shRNA Design. ShRNA and CRISPR Libraries. Webinar: RNAi & CRISPR Screens. User Manuals / PACs. Human Genome-Wide shRNA Library. For Genome-Wide Coverage in Human. 3 Modules, each covering approximately 6,500 genes. Each gene is targeted by 8 hairpins for a total of approximately 55,000 hairpins per module. Modules are made with non-overlapping barcodes so that they can be combined to form a complete genome-wide shRNA library. Top-ranking genes from the ...

cellecta.com

CRISPR Screening Services - Cellecta, Inc

https://www.cellecta.com/products-services/cellectas-complete-loss-of-function-screening-services/crispr-screening-services

KO / KD Validation. Expression & Reporter. RNAi and shRNA Design. ShRNA and CRISPR Libraries. Webinar: RNAi & CRISPR Screens. User Manuals / PACs. Cellecta can use the CRISPR/Cas9 system for screening. Start by using either the pooled CRISPR Human Genome Knockout Library. Or by using a Custom Pooled sgRNA Library. Identify Genes Essential for Growth and Proliferation. Identify genes essential for cell viability. Find targets that increase cell sensitivity to compounds or treatments. RNAi and shRNA Design.

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decipherpcp.eu

Mobile health for European citizens | Decipher

Skip to main content. Mobile health for European citizens. DECIPHER PCP project creates a mobile health care solution.The project aims to develop a mobile solution which enables secure cross-border access to existing patient healthcare portals. The European Commission publishes an evaluative study on the Cross-Border Healthcare Directive. DECIPHER was presented at the eHealth Week 2015 in Riga. DECIPHER Newsletter June 2015. PCP publications and guide books. Companies Interested in Participation.

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It's Hip to be Square. Christian Jean is an award-winning director, writer and executive producer who wants to capture your imagination. Race Owen is an accomplished actor, award winning executive producer and editor. Hobbies include technical wizardry. Witness the mysterious logo sting. If you stare at it long enough, you can read our minds. Don't say we didn't warn you. Click, if you dare. Here we are on the red carpet at The Other Venice Film Festival in Venice Beach, CA for the world premiere of.

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MaLam Decipher

Beyond WORDs.KeMbaLIKaN aKU Ke SyUrGa ITu. KitA BUkaN KiTa YaNG DuLu. BUkaN.JanGaN.BiArKaN. Saturday, March 9, 2013. Bicara apa kah itu. Buah fikiran, rasa perasaan. Kita punya masa depan. Bukan dengan rona kesedihan. Tak lagi sudi…. Tuk jasad jadi baying-bayang. Lirik dan lagu oleh:. Labels: Lirik dan Lagu. Friday, March 1, 2013. Sampai nanti kita pergi. Jalani hidup tak berarti. Akan ku teguh cintamu. Sungguh hidup dan matiku. Hanya…untukmu yang satu…. Hanya…untukmu yang satu…. Lagu and Lirik oleh;.

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Decipher Project

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decipherproject.net

The DECIPHER Project - An Open Source shRNA library RNAi Screening Platform

Bi-Specific Synthetic Lethality DDR shRNA Libraries. Developed under an NCI-supported synthetic lethality RNAi screening and DDR database contract research project. NCI Topic 290: siRNA Resource for Synthetic Lethal Screening of DNA Repair and Damage Signaling Networks. Please visit the Bi-Specific SL DDR shRNA Library Project Summary. Page for more information. DECIPHER Pooled Lentiviral shRNA Libraries. Page on the Cellecta website. DECIPHER Project members and customers can access DECIPHER Software.

decipherproject.org

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Decipher Publications to help crack the code of small busines success

Books that crack the code of success for small business owners! 51 Ironworks Hill Rd. Brookfield, CT 06804 USA. Welcome. Decipher Publications is the publishing arm of The Small Business Coach.com. Our first baby.er.booklet, Assess and Improve Your Company. Another publication, How to Get Rich Without Selling Your Soul. Can be used one-on-one or with groups. Assess and Improve Your Company. Every company, of any size, has to do only four things. 1 Please the owners. 2 Please the customers. To send me you...

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decruz's Journal

10 Feb 2008 11:13pm. HELLO HELLO HELLO (:. Over the past three weeks or so, many many assignments have finally been done and over with! Like the MEP animation that almost blinded my vision, multicamera production 1 2 and demo which went well, radio on-air assignment, CCC project, Broadcast co-hosting and mtv hosting and etc etc etc! Almost everything that has to be done for this semester, is done. All except one! And oh yes, Happy Chinese New Year! 27 Dec 2007 01:39am. 17th - 19th December. Ah, the love.

decipherr_.livejournal.com

decruz's Journal

10 Feb 2008 11:13pm. HELLO HELLO HELLO (:. Over the past three weeks or so, many many assignments have finally been done and over with! Like the MEP animation that almost blinded my vision, multicamera production 1 2 and demo which went well, radio on-air assignment, CCC project, Broadcast co-hosting and mtv hosting and etc etc etc! Almost everything that has to be done for this semester, is done. All except one! And oh yes, Happy Chinese New Year! 27 Dec 2007 01:39am. 17th - 19th December. Ah, the love.

decipherresearch.com

SIIM 2012 - Sunday Learning Series - Home

SIIM 2012 - Sunday Learning Series. Practical Natural Language Processing – Harvesting the Low Hanging Fruit. Participants should bring their own laptops to this session preloaded with lab software. The following software is needed to actively participate in the session:. VirtualBox appropriate for your operating system ( Windows. The NLP and Annotation tools you will be introduced to (available as a pre-loaded VirtualBox appliance). Analyze tasks that natural language processing can reliably handle now ...