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Photophosphorylation | Nirmal G's Blog

https://galinirmal.wordpress.com/2011/03/26/photophosphorylation

Nirmal G's Blog. The Information I Can…. Photosynthetic phosphorylation or photophosphorylation is the process of phosphate group transfer into ADP to synthesize energy rich ATP molecule making use of light as external energy source. According to chemi osmotic hypothesis (Mitchell 1961) the ATP is synthesized on ATPase complexes located on the non appressed portions of thylakoid membranes particularly towards margins. Non – Cyclic Photophosphorylation. The electrons released by P700 of PS-I in the presen...

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Normality for Solutions | Nirmal G's Blog

https://galinirmal.wordpress.com/2011/12/07/normality-for-solutions

Nirmal G's Blog. The Information I Can…. Find the Normality of a 70% pure O-Phosphoric acid (Specific Gravity – 1.54) of 100ml solution. Weight of O-Phosphoric acid is 70 x 1.54 = 107.8. Equivalent Weight is 98/3 = 32.6667. Normality = 107.8/(32.6667 x 0.1) = 33.0N. 1000 ml of solution contains 1540gm of phosphoric acid. Of this 70% mean 1078gm of phosphoric acid. Normality = 1078/32.6667 = 33.0N. Normality = Molarity x n (no. of hydrogen ions). By Nirmal Kumar G. December 7, 2011 at 06:02.

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Nucleic Acids Estimation – Types, Sensitivity & Interferences | Nirmal G's Blog

https://galinirmal.wordpress.com/2011/03/26/nucleic-acids-estimation-types-sensitivity-interferences

Nirmal G's Blog. The Information I Can…. Nucleic Acids Estimation – Types, Sensitivity & Interferences. Absorption of 260nm light by aromatic bases. Protein absorption may be corrected. A280/A260 indicates purity. Non-destructive. Measures both DNA and RNA. Enhancement of fluorescence of dye when bound to DNA. Not reliable with plasmids or small DNA. Does not measure RNA. May be used with crude extracts. Non-destructive. Gives no indication of purity. Measures single and double-stranded DNA and RNA.

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Protein Estimation – Types, Sensitivity & Effectors | Nirmal G's Blog

https://galinirmal.wordpress.com/2011/03/26/protein-estimation-types-sensitivity-effectors

Nirmal G's Blog. The Information I Can…. Protein Estimation – Types, Sensitivity & Effectors. Low        (1-20mg). Peptide bonds alkaline Cu. Zwitterionic buffers, some amino acids. Similar color with all proteins. Destructive to protein samples. 2Reduction of phosphomolybdate-phosphotunstate by Tyr and Trp. Ammonium sulphate, glycine, zwitterionic buffers, mercaptans. Time-consuming. Color varies with proteins. Critical timing of procedure. Destructive to protein samples. 2Copper complex with BCA; λ.

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