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Genome Resource Facility - GRF

The Genome Resource Facility (GRF) website is an interactive information centre relating to the practical use of next-generation sequencing and microarray-based technologies. Why has it been created? Who has created it? The GRF is a Faculty initiative founded by Dr Nick Dorrell and Professor Brendan Wren of the Pathogen Molecular Biology Department at LSHTM. The website authors and designers are Abdi Elmi and Ozan Gundogdu.

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The Genome Resource Facility (GRF) website is an interactive information centre relating to the practical use of next-generation sequencing and microarray-based technologies. Why has it been created? Who has created it? The GRF is a Faculty initiative founded by Dr Nick Dorrell and Professor Brendan Wren of the Pathogen Molecular Biology Department at LSHTM. The website authors and designers are Abdi Elmi and Ozan Gundogdu.
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Genome Resource Facility - GRF | grf.lshtm.ac.uk Reviews

https://grf.lshtm.ac.uk

The Genome Resource Facility (GRF) website is an interactive information centre relating to the practical use of next-generation sequencing and microarray-based technologies. Why has it been created? Who has created it? The GRF is a Faculty initiative founded by Dr Nick Dorrell and Professor Brendan Wren of the Pathogen Molecular Biology Department at LSHTM. The website authors and designers are Abdi Elmi and Ozan Gundogdu.

INTERNAL PAGES

grf.lshtm.ac.uk grf.lshtm.ac.uk
1

Genome Resource Facility

http://grf.lshtm.ac.uk/data_archive.htm

The data archive section is a page which holds accessible published data from publications within Prof. Brendan Wren's group.

2

Genome Resource Facility

http://grf.lshtm.ac.uk/links.htm

Bacterial Microarray Group at St George's (Bugs@S). European Bioinformatics Institute (EBI). Institute for Genomics and Bioinformatics - GRAZ. Microarray Gene Expression Data Society - MGED Society. Microarray Software Suite - TM4. National Center for Biotechnology Information (NCBI). PDBsum - Protein 3D structure database. The Arabidopsis Information Resource (TAIR). The Institute for Genomic Research (TIGR). The Wellcome Trust Sanger Institute. BASE - BioArray Software Environment.

3

Genome Resource Facility

http://grf.lshtm.ac.uk/microarraysection.htm

The microarray section describes the principles and methology behind microarray experiments. This section contains a general overview on the topic of microarray experiments followed by a more in-depth look at the various experimental stages. Each of the stages has a general overview and a basic protocol (HTML and pdf).

4

Genome Resource Facility

http://grf.lshtm.ac.uk/bioinformaticssection.htm

The Bioinformatics section describes software that is currently used to analyse microarray data e.g. ImaGene and GeneSpring. The section also contains a general overview on the topic of bioinformatics followed by a more in-depth overview of comparative genomics studies. The main bioinformatics software used within microarray data analysis are studied with an overview, a basic protocol and frequently asked questions.

5

Genome Resource Facility

http://grf.lshtm.ac.uk/sequencing.htm

However, increased bases sequenced at a reduced cost have always been desired. For this reason new theories were developed in the late 90’s. These have come to fruition in the last 5 years or so with advances in chemical and physical technology. Thus, we have now entered the next generation era of sequencing. Three main companies occupy the bulk of the next generation sequencing market. Figure 1. Emulsion PCR (ePCR). Figure 2. PCR amplification. Figure 3. PicoTitreTube. Figure 5. Light reaction. The appl...

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The Genome Resource Facility (GRF) website is an interactive information centre relating to the practical use of next-generation sequencing and microarray-based technologies. Why has it been created? Who has created it? The GRF is a Faculty initiative founded by Dr Nick Dorrell and Professor Brendan Wren of the Pathogen Molecular Biology Department at LSHTM. The website authors and designers are Abdi Elmi and Ozan Gundogdu.

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