Image Analyst MKII is an analysis tool for fluorescence time lapse microscopy. Extracts graphs of biological parameters from image sequences: ion concentrations, organelle shape changes and motion.
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Image Analyst MKII Online Manual | help.imageanalyst.net Reviews
https://help.imageanalyst.net
Image Analyst MKII is an analysis tool for fluorescence time lapse microscopy. Extracts graphs of biological parameters from image sequences: ion concentrations, organelle shape changes and motion.
help.imageanalyst.net
Workflow
http://help.imageanalyst.net/workflow_FixingArtifacts.html
Parametering image processing functions. Membrane Potential Calibration Wizard. Plotting intensities, ratios. Save or copy plot data in tabular format. Export RGB images or movies. Fixing imaging artifacts: Image Stabilizer, Channel Alignment, Spectral Unmixing and Lens Distortion. Image Stabilizer (Image registration in time lapses). To perform image registration (stabilization). Load a single or multi channel image recording. Align Series (Image Stabilizer). Select all images as Image (B. Set compare t...
Using Mathematica as scripting language
http://help.imageanalyst.net/workflow_mathematica.html
Parametering image processing functions. Membrane Potential Calibration Wizard. Plotting intensities, ratios. Save or copy plot data in tabular format. Export RGB images or movies. Using Mathematica as scripting language. This function enabled, but is not currently supported, and havent been tested with Mathematica versions above version 8. Contact support if interested in using Image Analyst MKII with Wolfram Research Mathematica. Image Analyst is linked to the Mathematica frontend through Mathlink.
Image Window Linkage
http://help.imageanalyst.net/workflow_linkage.html
Parametering image processing functions. Membrane Potential Calibration Wizard. Plotting intensities, ratios. Save or copy plot data in tabular format. Export RGB images or movies. Opening an experiment results one or more image windows (according to the number of channels or wavelengths) with identical x,y,t. Dimensions between the input images. Some image processing functions that require different sized inputs allow selecting non-linked Image Windows as Image B. Or use the Window menu. Toolbar button&...
Workflow
http://help.imageanalyst.net/workflow_pipeline_examples.html
Parametering image processing functions. Membrane Potential Calibration Wizard. Plotting intensities, ratios. Save or copy plot data in tabular format. Export RGB images or movies. Overlay, align and filter multi-channel image sequences and export as avi. Calculation of fluorescence intensity ratio plots after channel and series alignment and background subtraction, using automatic ROI drawing. Adding master parameters to a pipeline.
Workflow
http://help.imageanalyst.net/workflow_MembranePotentialCalibrationWizard.html
Parametering image processing functions. Membrane Potential Calibration Wizard. Plotting intensities, ratios. Save or copy plot data in tabular format. Export RGB images or movies. The Membrane Potential Calibration Wizard. The following step-by-step configuration of the Membrane Potential Calibration Wizard is given for the analysis of data recorded by the complete (known kP) calibration potentiometric protocol. Experimental paradigm for complete calibration with known kP:. Addition #4: 10 µ. Plasma mem...
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Image Analyst MKII Gallery
https://www.imageanalyst.net/imagegallery.html
Fluorescence microscopy image analysis. Automation - time series - physiology. Absolute and Unbiased ΔψM Assay. Online Help How To. Determination of the fraction of the cell occupied by mitochondria from confocal micrographs. Calibrated to electron microscopy. Instantaneous velocity vector measurment from pairs of images. Segment images and time lapses. Automate time lapse analysis for microplates. 43 Nova Ln, Novato, CA, 94945, USA. Cite as: "Image Analyst MKII (Image Analyst Software, Novato, CA)".
Image Analyst MKII Screenshots
https://www.imageanalyst.net/screenshots.html
Fluorescence microscopy image analysis. Automation - time series - physiology. Absolute and Unbiased ΔψM Assay. Online Help How To. Measurement of the absolute magnitude of the mitochondrial membrane potential. Calibration of plasma membrane potential, multiplexed with a GFP-based probe and post-hoc nulei staining. MitoSOX:Mitotracker Deep Red Ratio Calculation. Cell Tracking in Multiwell Plates. With senescent associated-beta-galactosidase (SA-BGAL) densitometry. 43 Nova Ln, Novato, CA, 94945, USA.
Absolute and Unbiased Calibration of Mitochondrial Membrane Potential
https://www.imageanalyst.net/calibrationwizard.html
Fluorescence microscopy image analysis. Automation - time series - physiology. Absolute and Unbiased ΔψM Assay. Online Help How To. Absolute and Unbiased ΔψM Assay. How absolute millivolts calculated. See how effects of ΔψP, cell and mitochondrial geometry and fluorescence background accounted for to calculate mV values. The Membrane Potential Calibration Wizard. This interactive dialog works hand in hand with the provided protocol to calibrate potentials without theoretical knowledge. In rat neurons ( 17.
Image Analyst MKII Pipelines
https://www.imageanalyst.net/pipelines.html
Fluorescence microscopy image analysis. Automation - time series - physiology. Absolute and Unbiased ΔψM Assay. Online Help How To. Built-in, menu-accessible pipelines support many basic and advanced image processing tasks and a variety of biological applications. See a list of pipelines here. Run the pipeline with a single click on a microscope stage position, microplate well or whole or partial microplates when using multi-dimensional image recordings. Optionally, master pipeline parameters can be asso...
Download Image Analyst MKII
https://www.imageanalyst.net/download.html
Fluorescence microscopy image analysis. Automation - time series - physiology. Absolute and Unbiased ΔψM Assay. Online Help How To. Download Image Analyst MKII. Download Version 3.2.0 (3.28.2016) 54MB. Fully functional 30-day trial version is available for download. Licensed customers please use the same link, and use your license key to permanently unlock the product. We strive for your security and privacy:. The installer is digitally signed by "Image Analyst Software". Your connection is SSL encrypted.
Image Analyst Software Press
https://www.imageanalyst.net/press.html
Fluorescence microscopy image analysis. Automation - time series - physiology. Absolute and Unbiased ΔψM Assay. Online Help How To. An imbalance of cellular bioenergetics in pancreatic beta-cells is linked to type 2 diabetes. Novato, CA/July 28, 2015:. Impaired activation of mitochondrial energy metabolism in the presence of glucose has been demonstrated in pancreatic beta-cells from patients with type 2 diabetes. The cause of this dysfunction has been unknown. Publishing online in Endocrinology. Observa...
Advanced Biological Image Processing
https://www.imageanalyst.net/advanced_image_processing.html
Fluorescence microscopy image analysis. Automation - time series - physiology. Absolute and Unbiased ΔψM Assay. Online Help How To. Advanced Biological Image Processing. Advanced Image Processing for Time-Lapse Microscopy Image Analyst MKII provides tools both for routine manual and robust automated analysis of fluorescence time-lapse microscopic recordings. Most of the functionalities highlighted below are also incorporated into ready-to-use pipelines. Fastest available template matching algorithm (comp...
Bibliography
https://www.imageanalyst.net/references.html
Fluorescence microscopy image analysis. Automation - time series - physiology. Absolute and Unbiased ΔψM Assay. Online Help How To. Image Analyst MKII or algorithms implemented in Image Analyst MKII have been used in the following publications:. Gerencser, A.A. And Adam-Vizi, V. 2001) Selective, High-Resolution Fluorescence Imaging of Mitochondrial Ca2 Concentration. Cell Calcium. 30.5:311-321. Gerencser AA, Doczi J, Fiskum G, Adam-Vizi V. J Neurochem. 2004 Oct;91(2):471 83. Gerencser, A.A.,. Gerencser A...
Image Analyst MKII
https://www.imageanalyst.net/buy.html
Fluorescence microscopy image analysis. Automation - time series - physiology. Absolute and Unbiased ΔψM Assay. Online Help How To. Cell Tracking in Microplate Data. Reactive Oxygen Species Analysis. Image Analyst MKII supports a range of fluorescence microscopic imaging assays by generic protocols for data recording on typical microscopy systems and specific analysis protocols using pre-configured pipelines accessible from the main menu. Measurment in absolute millivolts. With simple pipeline design.
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Image Analyst MKII Online Manual
Getting started and more - the workflow in Image Analyst MKII. Open Image sequences of one or more gray value channels. Importing images with Bio-Formats. Basic and advanced image processing functions. Opening multiple TIF files in folders. Determine or Assign Microplate Well Names to Positions. Setup DFT Filter and Optimizations. Attach Intensity Gating (IMD Intensity. Membrane Potential Calibration Wizard. Crossbleed correction factor calculation. Smoothing and optimizing the look of images. Protocol f...
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