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Today's science fiction is tomorrow's science fact. Saturday, November 7, 2009. Done by: Janice Yeh. This is my second last department. Mainly observation of the work flow only.wasn't allow to do as i was only posted there for a week. 1) The following photos are taken with permission of the department supervisor. 2) Photos below may be abit disgusting. BE PREPARED! 1)When specimens are receieved, they are soaked fully in formalin to remove the smell and for preservation. Once the stain is done, cutting o...

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MedScientist | med-scientist-in-action.blogspot.com Reviews

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Today's science fiction is tomorrow's science fact. Saturday, November 7, 2009. Done by: Janice Yeh. This is my second last department. Mainly observation of the work flow only.wasn't allow to do as i was only posted there for a week. 1) The following photos are taken with permission of the department supervisor. 2) Photos below may be abit disgusting. BE PREPARED! 1)When specimens are receieved, they are soaked fully in formalin to remove the smell and for preservation. Once the stain is done, cutting o...

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MedScientist | med-scientist-in-action.blogspot.com Reviews

https://med-scientist-in-action.blogspot.com

Today's science fiction is tomorrow's science fact. Saturday, November 7, 2009. Done by: Janice Yeh. This is my second last department. Mainly observation of the work flow only.wasn't allow to do as i was only posted there for a week. 1) The following photos are taken with permission of the department supervisor. 2) Photos below may be abit disgusting. BE PREPARED! 1)When specimens are receieved, they are soaked fully in formalin to remove the smell and for preservation. Once the stain is done, cutting o...

INTERNAL PAGES

med-scientist-in-action.blogspot.com

1

MedScientist: Transformation of chemically ultra-competent E.coli with plasmid DNA

http://med-scientist-in-action.blogspot.com/2009/09/transformation-of-chemically-ultra.html

Today's science fiction is tomorrow's science fact. Monday, September 21, 2009. Transformation of chemically ultra-competent E.coli with plasmid DNA. Posted by Vo Thu Hong Anh [Jess] 0705364H. To generate a large amount of plasmid DNA containing a desired gene, with the aid of chemically ultra-competent E.coli. Chemically ultra-competent E.coli. Cells will also be heated at 42. Place the paper disc containing plasmid DNA (commercially made and shipped) into a 1.5mL-centrifuge tube. On ice to thaw. After ...

2

MedScientist: Slide-staining

http://med-scientist-in-action.blogspot.com/2009/10/slide-staining.html

Today's science fiction is tomorrow's science fact. Wednesday, October 21, 2009. Greetings to everyone, :). My previous post was about slide-making, the process of dropping cells onto the slides for observation of chromosomes under phase contrast microscope. I will be explaining the process of slide-staining after dropping slides. Contents of my post. 1) Method for slide-staining. It is the process of staining slides to observe chromosomes under microscope. Wright’s stain - 8ml. Coplin Jars - 4 jars.

3

MedScientist: October 2009

http://med-scientist-in-action.blogspot.com/2009_10_01_archive.html

Today's science fiction is tomorrow's science fact. Wednesday, October 28, 2009. Processing of liver tissue with liquid nitrogen. Posted by: Vo Thu Hong Anh [Jess] 0750364H. To grind a piece of liver tissue into powder so that RNA could be extracted. Liver tissue is immersed in liquid nitrogen to become frozen; hence, it can be broken up and grinded into powder. Also, at this condition, RNA degradation is prevented too. Pour liquid nitrogen into the mortar. Immediately place the tube back on dry ice.

4

MedScientist: Immunochemistry- G6PD Quantitative Screening Test

http://med-scientist-in-action.blogspot.com/2009/08/immunochemistry-g6pd-quantitative.html

Today's science fiction is tomorrow's science fact. Monday, August 24, 2009. Immunochemistry- G6PD Quantitative Screening Test. Its my turn to post once again, woo! Today I’m gonna share with you guys, the Glucose-6-phosphate dehydrogenase (G6PD) screening test (Quantitative). By the OSMMR-D-D assay, which is a new machine currently under evaluation by the Immunochemistry lab. (which is the lab I’m permanently attached to.). End of story, good bye! Joanna Yeo, woo! MedScientists of Grp 6. Hope you are we...

5

MedScientist: September 2009

http://med-scientist-in-action.blogspot.com/2009_09_01_archive.html

Today's science fiction is tomorrow's science fact. Wednesday, September 30, 2009. Its my turn again. i'm gonna share with you one of the test that is carried out by the Architect i2000SR immunoassay. The Rubella IgG test. It is measure colourmetrically as RLUs and this RLUs detected by optical system is directly proportional to concentration of rubella Ab present in serum. The serum left is kept for 1 year at -70 to -90 degrees celsius for traceability. =). Done by: Joanna Yeo. Admin No. 0702054H. Vener...

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bordetellas.blogspot.com

Bordetellas Logbook:]: July 2009

http://bordetellas.blogspot.com/2009_07_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Tuesday, July 28, 2009. Hey Siti Shahimah is here again. :). Okay, so I have learnt about TDx/IMX. Basically, TDx/IMX are the name of analyzers that help to detect therapeutic dosage of drugs so as to ensure appropriate therapy for patients. TDx analyzes drugs such as cyclosporine, methotrexate and everolimus. Cyclosporine and everolimus are immunosuppresant drugs whereas methotrexate is an antineoplastic drug. 150 uL of pat...

bordetellas.blogspot.com

Bordetellas Logbook:]: September 2009

http://bordetellas.blogspot.com/2009_09_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Saturday, September 26, 2009. For hematology section, tests such as full blood count (FBC), erythrocyte sedimentation rate ESR), peripheral blood films (PBF), and retic count are done. there are also malaria tests and dengue panel. Sunday, September 13, 2009. General theory of using an organic solvent to precipitate out proteins in plasma:. Organic solvent reduces water activity. This is done by:. 5 Vortex for 2 minutes.

bordetellas.blogspot.com

Bordetellas Logbook:]: blood collection

http://bordetellas.blogspot.com/2009/10/blood-collection.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Sunday, October 11, 2009. In this post, I am going to write about a tutorial given by one of our colleague. All of us have to attend this tutorials unless we are busy at our work stations. These tutorials are given at least once every 2 weeks. Topic for today: blood collection. Times to provide thorough mixing of the additives. Also, it is important to note that shaking of blood tubes may cause hemolysis of the blood. They c...

bordetellas.blogspot.com

Bordetellas Logbook:]: ways to protect the column from damaging

http://bordetellas.blogspot.com/2009/11/ways-to-protect-column-from-damaging.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Thursday, November 12, 2009. Ways to protect the column from damaging. Sorry for the super late post! Since the column of the HPLC is very expensive, we can have to do things to maximize the length of its life:). Here are the things to do:. During shutdown, always flush the column with ultrapure water to remove all the salts present from the buffer. If this is not done, salts will precipitate and cause clogging of column.

bordetellas.blogspot.com

Bordetellas Logbook:]: Immunofixation Electrophoresis

http://bordetellas.blogspot.com/2009/10/immunofixation-electrophoresis.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Friday, October 16, 2009. 1 Proteins in the samples (urine and serum) are separated by electrophoresis on agarose gel. 2 Immunofixation of electrophoresed proteins includes, the monospecific antisera diffuse into. Agarose gel and bind to the antigens (proteins), if present. Thus, resulting in the precipitation. 3 The unprecipitated antigens are removed from the gel by blotting and washing. Of the fractions is dark in colour.

wedonknow.blogspot.com

wedonknow.blogspot.com (:: G6PD qualitative screening test

http://wedonknow.blogspot.com/2009/09/g6pd-qualitative-screening-test.html

Wedonknow.blogspot.com (:. Wednesday, September 30, 2009. G6PD qualitative screening test. G6PD is a key enzyme in the hexose monophosphate pathway (HMP) and is required for the formation of NADPH. NADPH is essential for maintaining the integrity of the erythrocyte membrane. Specimen collection and preparation. Process specimen collected in EDTA tube. Before analysis, check that the specimen is not clotted. Performing G6PD screening test by R&D diagnostic G6PD deficiency screening test kit. With the same...

wedonknow.blogspot.com

wedonknow.blogspot.com (:: Immunochemistry- G6PD quantification

http://wedonknow.blogspot.com/2009/10/immunochemistry-g6pd-quantification.html

Wedonknow.blogspot.com (:. Monday, October 26, 2009. Hey guys, sorry for the late posting! This will be the last time I'm sharing! This time I am going to talk about the quantitative determination of G6PD on an analyzer, which is currently undergoing evaulation. Why do this test? An awareness of G6PD deficiency had been raised many years back in Singapore. Hence, all newborns will be screened for this deficiency. And of course, the samples used for this test will be all cord blood samples. TG02 - Group 8.

bordetellas.blogspot.com

Bordetellas Logbook:]: October 2009

http://bordetellas.blogspot.com/2009_10_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Sunday, October 25, 2009. Human Pancreatic Polypeptide (PP). Human pancreatic polypeptide (PP) is a polypeptide hormone secreted by the pancreatic polypeptide (PP) cells of the islets of Langerhans in the endocrine portion of the pancreas. It consists of a chain of 36 amino acids. 1 Capture of human PP molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-PP antibody. 7 Quantificati...

emadtechs.blogspot.com

emadtechs: Slide Making

http://emadtechs.blogspot.com/2009/10/slide-making.html

Sunday, October 25, 2009. Hi everyone, I’m Justin here. Sorry for my super late post. For the last post, I’ll write about slide making. The lab I’m in uses a de-humidifier and the air conditioner for temperature and humidity. Another lab has a slide drying chamber, in which temperature, humidity, as well as air flow can be controlled more easily. It is important to consistently get slides of appropriate quality. That’s all. Take care. To SIP and MP! Ng Tze Yang Justin. October 26, 2009 at 10:11 AM. The f...

emadtechs.blogspot.com

emadtechs: October 2009

http://emadtechs.blogspot.com/2009_10_01_archive.html

Sunday, October 25, 2009. Hi everyone, I’m Justin here. Sorry for my super late post. For the last post, I’ll write about slide making. The lab I’m in uses a de-humidifier and the air conditioner for temperature and humidity. Another lab has a slide drying chamber, in which temperature, humidity, as well as air flow can be controlled more easily. It is important to consistently get slides of appropriate quality. That’s all. Take care. To SIP and MP! Ng Tze Yang Justin. Sunday, October 18, 2009. 10 10ml o...

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MedScientist

Today's science fiction is tomorrow's science fact. Saturday, November 7, 2009. Done by: Janice Yeh. This is my second last department. Mainly observation of the work flow only.wasn't allow to do as i was only posted there for a week. 1) The following photos are taken with permission of the department supervisor. 2) Photos below may be abit disgusting. BE PREPARED! 1)When specimens are receieved, they are soaked fully in formalin to remove the smell and for preservation. Once the stain is done, cutting o...

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