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molecular beaconsDesigned by Salvatore A.E. Marras
http://www.molecular-beacons.org/
Designed by Salvatore A.E. Marras
http://www.molecular-beacons.org/
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molecular beacons | molecular-beacons.org Reviews
https://molecular-beacons.org
Designed by Salvatore A.E. Marras
molecular beacons
http://www.molecular-beacons.org/MB_publications.html
Basic papers on molecular beacons. Review papers on molecular beacons. Papers on molecular beacons used for detection of DNA and RNA in living cells. Papers on molecular beacons used in real-time PCR for SNP. And mutation detection assays. Papers on molecular beacons used in real-time PCR for DNA, RNA. And pathogenic detection assays. Papers on molecular beacons used in real-time NASBA assays. Papers on molecular beacons used on arrays. Papers on general application of molecular beacons. Ortiz E, Estrada...
molecular beacons
http://www.molecular-beacons.org/MB_array.html
Self-reporting Microarray Platforms that use. We developed a novel platform in which molecular beacons are immobilized within highly hydrated microhydrogels, creating a local environment that mimics solution-based hybridization events, and does not compromise the inherently performance of molecular beacon probes. This work appeared in the March 23, 2012, issue of Soft Matter (Volume 8, Number 11, pages 3067-3076) and the cover of the journal featured our publication. Recent Publications from Our Group.
molecular beacons
http://www.molecular-beacons.org/MB_SC_FAQ.html
The manual conjugation of a fluorophore or a quencher to an oligo resulted in a low yield. 150; Check the pH of the buffers used in the coupling reactions and use fresh dyes. The reactive dyes should be stored at -20 °C in the presence of a desiccant. 150; For the conjugation of a fluorophore to a trityl-protected thiol group, prior to performing the coupling reactions, prepare fresh solutions of both silver nitrate and dithiothreitol. What are the best ways to store a molecular beacon preparation? After...
molecular beacons
http://www.molecular-beacons.org/MB_introduction.html
Introduction on Molecular Beacons. To read more on molecular beacons. Recent Publications from Our Group. Vargas DY, Kramer FR, Tyagi S, and Marras SAE. (2016) Multiplex real-time PCR assays that measure the abundance of extremely rare mutations associated with cancer. PLoS ONE 11, e0156546. To access this article. Public Health Research Institute. New Jersey Medical School - Rutgers, The State University of New Jersey.
molecular beacons
http://www.molecular-beacons.org/MB_SC_protocol.html
Synthesis and Characterization of Molecular Beacons. Characterization of molecular beacons. Real-time monitoring of polymerase chain reactions. 150; High-pressure liquid chromatograph. 150; Spectrofluorometric thermal cycler with a capacity to monitor fluorescence in real time. 150; 0.1 M sodium bicarbonate, pH 8.5. 150; 0.2 M sodium bicarbonate, pH 9.0. 150; HPLC Buffer A: 0.1 M triethylammonium acetate, pH 6.5, filtered and degassed. 150; TE buffer: 1 mM EDTA, 10 mM Tris-HCl, pH 8.0. To top of page.
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Gene Quantification & real-time PCR dyes and chemistries
http://www.gmo-qpcr-analysis.info/chemistry.html
Dyes and Fluorescence detection chemistry in qPCR. Short summary of detection formats. HRM - High Resolution Melt Dyes. Amplifluor Quantitative PCR Detection System. LUX (Light Upon eXtension) primers. The iCycler iQ Detection System. For multiplex real-time PCR Assays. A novel real-time quantitative PCR method using attached universal template probe. SYBR Green based Quantitative PCR. Flexible – SYBR Green JumpStart Taq ReadyMixes for SYBR based QPCR are formulated with MgCl2 or packaged with a se...
Links | Laboratory of Biology & Molecular Virology
http://www.kostrikislab.com/links
Laboratory of Biotechnology and Molecular Virology. Cyprus Ministry of Health. Http:/ www.moh.gov.cy/moh/moh.nsf/index en/index en. Http:/ hcv.lanl.gov/content/hcv-index. Http:/ www.hiv.lanl.gov/content/index. HIV Drug Resistance Database. Http:/ hivdb.stanford.edu/index.html. Hellenic Accreditation System S.A. (ESYD). Http:/ www.esyd.gr/eng/index.php. Http:/ www.molecular-beacons.org/. Http:/ www.ncbi.nlm.nih.gov/pubmed/. Quality Control for Molecular Diagnostics. Http:/ www.qcmd.org.
Gene Quantification & real-time PCR dyes and chemistries
http://www.gene-quantification.com/chemistry.html
Dyes and Fluorescence detection chemistry in qPCR. Short summary of detection formats. HRM - High Resolution Melt Dyes. Amplifluor Quantitative PCR Detection System. LUX (Light Upon eXtension) primers. The iCycler iQ Detection System. For multiplex real-time PCR Assays. A novel real-time quantitative PCR method using attached universal template probe. SYBR Green based Quantitative PCR. Flexible – SYBR Green JumpStart Taq ReadyMixes for SYBR based QPCR are formulated with MgCl2 or packaged with a se...
Gene Quantification & real-time PCR dyes and chemistries
http://www.gene-quantification.net/chemistry.html
Dyes and Fluorescence detection chemistry in qPCR. Short summary of detection formats. HRM - High Resolution Melt Dyes. Amplifluor Quantitative PCR Detection System. LUX (Light Upon eXtension) primers. The iCycler iQ Detection System. For multiplex real-time PCR Assays. A novel real-time quantitative PCR method using attached universal template probe. SYBR Green based Quantitative PCR. Flexible – SYBR Green JumpStart Taq ReadyMixes for SYBR based QPCR are formulated with MgCl2 or packaged with a se...
Gene Quantification & real-time PCR dyes and chemistries
http://www.qpcrupdate.info/chemistry.html
Dyes and Fluorescence detection chemistry in qPCR. Short summary of detection formats. HRM - High Resolution Melt Dyes. Amplifluor Quantitative PCR Detection System. LUX (Light Upon eXtension) primers. The iCycler iQ Detection System. For multiplex real-time PCR Assays. A novel real-time quantitative PCR method using attached universal template probe. SYBR Green based Quantitative PCR. Flexible – SYBR Green JumpStart Taq ReadyMixes for SYBR based QPCR are formulated with MgCl2 or packaged with a se...
Gene Quantification & real-time PCR dyes and chemistries
http://www.gmo-qpcr-analysis.com/chemistry.html
Dyes and Fluorescence detection chemistry in qPCR. Short summary of detection formats. HRM - High Resolution Melt Dyes. Amplifluor Quantitative PCR Detection System. LUX (Light Upon eXtension) primers. The iCycler iQ Detection System. For multiplex real-time PCR Assays. A novel real-time quantitative PCR method using attached universal template probe. SYBR Green based Quantitative PCR. Flexible – SYBR Green JumpStart Taq ReadyMixes for SYBR based QPCR are formulated with MgCl2 or packaged with a se...
biomers.net | Real time PCR Probes - biomers.net Oligonucleotides
http://www.biomers.net/en/products/dna/real_time_pcr_probes.html
Https:/ webstore.biomers.net/BiomersTimeWebService.asmx. Between 7 am and 7 pm for all and any questions around your oligonucleotides call:. 49 731 70396 0. 49 731 70396 11. We help you with everything around oligonucleotides - fast, competently and reliably! DGHM annual meeting takes place in Ulm from 11.09.-14.09.2016. Visit us at our booth! NEW: discounts for RNA. For the scales RS27 und RS40. From 45,89 EUR / 41,40 GBP per oligo in scale RS27, HPLC purification included. QPCR - new BMN quenchers.
Dyes/Quenchers | Ask An Expert
https://trilinkbiotech.wordpress.com/oligos/dyesquenchers
TriLink BioTechnologies: The Modified Nucleic Acid Experts. Can I have a dye conjugated to my oligonucleotide internally? Yes We use the succinimidyl ester version of the dye and conjugate it to a selective placement linker. We then post-synthetically conjugate the dye to the oligonucleotide. Other options include using a dT-FAM or dT-TAMRA. These dyes are already conjugated to the dT-C6 amino linker. What is the difference between a 6-FAM and FITC labeled oligonucleotide? How does a quencher work? What ...
biomers.net | Real time PCR Probes - biomers.net Oligonucleotides
http://www.bio-mers.net/en/products/dna/real_time_pcr_probes.html
Https:/ webstore.biomers.net/BiomersTimeWebService.asmx. Between 7 am and 7 pm for all and any questions around your oligonucleotides call:. 49 731 70396 0. 49 731 70396 11. We help you with everything around oligonucleotides - fast, competently and reliably! DGHM annual meeting takes place in Ulm from 11.09.-14.09.2016. Visit us at our booth! NEW: discounts for RNA. For the scales RS27 und RS40. From 45,89 EUR / 41,40 GBP per oligo in scale RS27, HPLC purification included. QPCR - new BMN quenchers.
Gene Quantification & real-time PCR dyes and chemistries
http://www.gene-quantification.org/chemistry.html
Dyes and Fluorescence detection chemistry in qPCR. Short summary of detection formats. HRM - High Resolution Melt Dyes. Amplifluor Quantitative PCR Detection System. LUX (Light Upon eXtension) primers. The iCycler iQ Detection System. For multiplex real-time PCR Assays. A novel real-time quantitative PCR method using attached universal template probe. SYBR Green based Quantitative PCR. Flexible – SYBR Green JumpStart Taq ReadyMixes for SYBR based QPCR are formulated with MgCl2 or packaged with a se...
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www.molecular-assembly.com
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Home - Molecular Basis of Life
49 69 660567 0. X69;nfo@molecular-basis-of-life.org. Publishing and Peer-review insiders’ perspectives. Publishing and Peer-review insiders’ perspectives. Molecular Basis of Life 2017 - International Fall Meeting of the German Society for Biochemistry and Molecular Biology. September 24 - 27, 2017 / Ruhr University Bochum. Molecular Basis of Life 2017 - International Fall Meeting of the German Society for Biochemistry and Molecular Biology. September 24 - 27, 2017 / Ruhr University Bochum.
molecular beacons
Recent Publications from our group. Schlachter S, Chan K, Marras SAE, and Parveen N (2017) Detection and differentiation of lyme spirochetes and other tick-borne pathogens from blood using real-time PCR with molecular beacons. Methods in Molecular Biology 1616: 155-170. Vargas DY, Kramer FR, Tyagi S, and Marras SAE. (2016) Multiplex real-time PCR assays that measure the abundance of extremely rare mutations associated with cancer. PLoS ONE 11, e0156546. To access this article.
molecular beacons
Recent Publications from our group. Schlachter S, Chan K, Marras SAE, and Parveen N (2017) Detection and differentiation of lyme spirochetes and other tick-borne pathogens from blood using real-time PCR with molecular beacons. Methods in Molecular Biology 1616: 155-170. Vargas DY, Kramer FR, Tyagi S, and Marras SAE. (2016) Multiplex real-time PCR assays that measure the abundance of extremely rare mutations associated with cancer. PLoS ONE 11, e0156546. To access this article.
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www.qPCR.asia & www.real-time-PCR.asia & www.Gene-Quantification.asia
WwwqPCR.asia and www.real-time-PCR.asia and www.Gene-Quantification.asia - Gene-Quantification.asia - The reference in qPCR - Academic and Industrial Information Platform - molecular-biology.asia.
Molecular Biology Info Server
Molecular Biology Info Server - Main focus of GENE QUANTIFICATION web page is to describe and summarize all technical aspects involved in quantitative gene expression analysis using real-time RT-PCR and competitive RT-PCR. It illustrates the usefulness of absolute and relative quantification assays in real-time PCR and real-time RT-PCR - molecular-biology.info.
Molecular Biology Resources
Useful guides and resources for molecular biologists. Tips for improving Sanger DNA sequencing. Exonuclease I - Shrimp Alkaline Phosphatase Clean Up of PCR Products. Reviews of DNA sequencing services. UCLA DNA sequencing core facility. University of Maine DNA sequencing core facility. DF/HCC DNA Core Facility. Washington University SNP Research Facility. Gene Link DNA sequencing. Stony Brook University DNA Core Facility. Tufts University DNA Core Facility. Qiagen DNA Sequencing service. DNA sequencing t...