proteinfacility.nki.nl

NKI Protein Facility

Protein Facility of The Netherlands Cancer Institute (NKI) Amsterdam. The NKI Protein Facility provides support for the production, purification characterisation and crystallisation of proteins. It is a National Facility supported by NKI and NWO and and is accessible to both internal. The facility is located at the top floor of the B-building of the NKI (B8) and shares its lab space and equipment with the Structural Biology groups of Anastassis Perrakis and Titia Sixma. For more details please visit the ...

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NKI Protein Facility | proteinfacility.nki.nl Reviews

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Protein Facility of The Netherlands Cancer Institute (NKI) Amsterdam. The NKI Protein Facility provides support for the production, purification characterisation and crystallisation of proteins. It is a National Facility supported by NKI and NWO and and is accessible to both internal. The facility is located at the top floor of the B-building of the NKI (B8) and shares its lab space and equipment with the Structural Biology groups of Anastassis Perrakis and Titia Sixma. For more details please visit the ...

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NKI Protein Facility | proteinfacility.nki.nl Reviews

https://proteinfacility.nki.nl

Protein Facility of The Netherlands Cancer Institute (NKI) Amsterdam. The NKI Protein Facility provides support for the production, purification characterisation and crystallisation of proteins. It is a National Facility supported by NKI and NWO and and is accessible to both internal. The facility is located at the top floor of the B-building of the NKI (B8) and shares its lab space and equipment with the Structural Biology groups of Anastassis Perrakis and Titia Sixma. For more details please visit the ...

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proteinfacility.nki.nl

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SupportandServices

http://proteinfacility.nki.nl/services.htm

Protein Facility Support and Services. The NKI Protein Facility is open available for Academic scientists. Both NKI- as well as external scientists can apply for projects. A list of support and services is given below. For more details please check the individual pages. If your request is not available from the list, please contact. Us to discuss the possibilities. Initial experiments can be performed by the Facility members, however the aim of the facility is to assist. Mammalian expression system, usin...

2

Crystallisation

http://proteinfacility.nki.nl/crystallisation.htm

Crystal Screening - Nanoliter volumes. The Facility has access to a high-throughput macromolecular crystallisation platform. Liquid handling robots are available for preparation of nanoliter crystallisation drops in 96-well format, including a 96-well pipetting robot (Hydra II) for filling up reservoir wells and two Mosquito's for creating nanoliter crystallisation drops. Preparation of a single 96-well plate takes generally about 5 minutes. Crystal Screening - Microfluidics. Due to the small size of the...

3

Mass Spectrometry

http://proteinfacility.nki.nl/mass_spectrometry.htm

An LTQ-Orbitrap Discovery mass spectrometer (Thermo Scientific) coupled to a nano-LC system (Proxeon) has been installed in 2010. The instrument is used for Protein identification, validation and limited posttranslational modifications. The mass spec is used for protein quality control and to confirm that the purified protein is indeed the correct protein.For this purpose either purified proteins or protein bands from SDS gels can be used for the analysis. NKI Protein Facility Last updated: 18 April 2012.

4

Expression Platforms

http://proteinfacility.nki.nl/expression_platforms.htm

Depending on the project, the platform for expression will be chosen. Proteins can be expressed in E. coli, Insect cells and mammalian cells. For each of the systems we can do small scale (few ml) as well as large scale (few liters) production. Expression trials can be performed in volumes as low as 2 ml (in 24-well format). Incubators are available for large scale expression (up to 6 Liter/ incubator) at variable temperatures. Our mammalian expression system has currently been optimized for HEK-293T cel...

5

Expression_screening

http://proteinfacility.nki.nl/expression_screening.htm

High-Throughput Protein Expression Screening. The Facility has acquired a customized Tecan Evo system for high-throughput protein expression screning in 24-well plates. Various conditions like E. coli strain, lysis buffer and expression construct can be compared. NKI Protein Facility Last updated: 13 April 2012.

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Perrakis, A. Group - Netherlands Cancer Institute

http://www.nki.nl/divisions/biochemistry/perrakis-a-group

Ga direct naar de inhoud. Biochemistry: Anastassis (Tassos) Perrakis. Anastassis (Tassos) Perrakis, Ph.D. Anastassis (Tassos ) Perrakis. About Anastassis (Tassos) Perrakis. Research interest: Macromolecular Structures. I have been involved in many methodology oriented initiatives, providing scientific developments that enable specific software tools in determining macromolecular structures better and faster. For optimizing "working models" before they are submitted to the PDB. Autotaxin is a secreted pho...

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proteinfacility.nki.nl

NKI Protein Facility

Protein Facility of The Netherlands Cancer Institute (NKI) Amsterdam. The NKI Protein Facility provides support for the production, purification characterisation and crystallisation of proteins. It is a National Facility supported by NKI and NWO and and is accessible to both internal. The facility is located at the top floor of the B-building of the NKI (B8) and shares its lab space and equipment with the Structural Biology groups of Anastassis Perrakis and Titia Sixma. For more details please visit the ...

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