crispr.dbcls.jp

CRISPRdirect

Mdash; Rational design of CRISPR/Cas target. Or Paste a nucleotide sequence:. Sample sequence atgccgcgcgtcgtgcccgaccagagaagcaagttcgagaacgaggagttttttaggaag ctgagccgcgagtgtgagattaagtacacgggcttcagggaccggccccacgaggaacgc caggcacgcttccagaacgcctgccgcgacggccgctcggaaatcgcttttgtggccaca ggaaccaatctgtctctccagttttttccggccagctggcagggagaacagcgacaaaca cctagccgagagtatgtcgacttagaaagagaagcaggcaaggtatatttgaaggctccc atgattctgaatggagtctgtgttatctggaaaggctggattgatctccaaagactggat ggtatgggctgtctggagtttgatgaggagcgagcccagcaggaggatg...

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CRISPRdirect | crispr.dbcls.jp Reviews

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Mdash; Rational design of CRISPR/Cas target. Or Paste a nucleotide sequence:. Sample sequence atgccgcgcgtcgtgcccgaccagagaagcaagttcgagaacgaggagttttttaggaag ctgagccgcgagtgtgagattaagtacacgggcttcagggaccggccccacgaggaacgc caggcacgcttccagaacgcctgccgcgacggccgctcggaaatcgcttttgtggccaca ggaaccaatctgtctctccagttttttccggccagctggcagggagaacagcgacaaaca cctagccgagagtatgtcgacttagaaagagaagcaggcaaggtatatttgaaggctccc atgattctgaatggagtctgtgttatctggaaaggctggattgatctccaaagactggat ggtatgggctgtctggagtttgatgaggagcgagcccagcaggaggatg...

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1 pam sequence requirement

2 specificity check

3 none

4 whats' new

5 full text

6 news archives

7 crisprdirect

8 dbcls

9 bioinformatics

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CRISPRdirect | crispr.dbcls.jp Reviews

https://crispr.dbcls.jp

Mdash; Rational design of CRISPR/Cas target. Or Paste a nucleotide sequence:. Sample sequence atgccgcgcgtcgtgcccgaccagagaagcaagttcgagaacgaggagttttttaggaag ctgagccgcgagtgtgagattaagtacacgggcttcagggaccggccccacgaggaacgc caggcacgcttccagaacgcctgccgcgacggccgctcggaaatcgcttttgtggccaca ggaaccaatctgtctctccagttttttccggccagctggcagggagaacagcgacaaaca cctagccgagagtatgtcgacttagaaagagaagcaggcaaggtatatttgaaggctccc atgattctgaatggagtctgtgttatctggaaaggctggattgatctccaaagactggat ggtatgggctgtctggagtttgatgaggagcgagcccagcaggaggatg...

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crispr.dbcls.jp

1

CRISPRdirect help

http://crispr.dbcls.jp/doc

Mdash; Rational design of CRISPR/Cas target. CRISPRdirect is a web server for selecting rational CRISPR/Cas targets from an input sequence. All services of the CRISPRdirect web server are provided free of charge to all users. Acknowledgement: We thank Ms. Junko Morita for making the tutorial video on TogoTV. Example 1 Selecting target sites for human B melanoma antigen (BAGE) gene. Enter the accession number for human BAGE gene (NM 001187). Click 'retrieve sequence' to get nucleotide sequence from GenBank.

2

CRISPRdirect help

http://crispr.dbcls.jp/doc/pam.html

An arbitrary 3 nt sequence using IUB codes can be specified in ( 5. NRG (= NGG or NAG). IUB codes for nucleic acid bases.

3

CRISPRdirect help

http://crispr.dbcls.jp/doc/upload.html

Uploading a sequence file. You can upload a sequence file using upload form ( 4. The file should be plain text, not MS Word .doc file. FASTA format or a plain nucleotide sequence up to 10 kbp is accepted.

4

CRISPRdirect help

http://crispr.dbcls.jp/doc/input.html

You can directly paste a nucleotide sequence into text field ( 3. FASTA format or a plain nucleotide sequence up to 10 kbp is accepted.

5

CRISPRdirect help

http://crispr.dbcls.jp/doc/selectdb.html

Select species from the drop-down list ( 6. The number of target sites (including both on-target and off-target sites) in the selected species is displayed. The full list of supported organisms and data sources is available on the help page. Illustrated overview of specificity check.

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rc.rois.ac.jp

プレスリリース | データ中心科学リサーチコモンズ Data Centric Science Research Commons

http://rc.rois.ac.jp/report/press.html

プレスリリース NBDC RDFポータル運用開始. このコケを氷河コケ無性芽集合体 glacial moss gemmae aggregation: GMGA 画像参照と名付け、形態的および遺伝子的な特徴から、南極でも報告されているコケ類 Ceratodon purpureus と断定することができました。植竹淳新領域融合研究センター国立極地研究所融合プロジェクト特任研究員. 掲載誌はこちら米国科学雑誌 PLOS ONE. プロジェクト名遺伝機能生命システム. 掲載誌はこちら米国科学雑誌 PLoS Genetics. 解説はこちら遺伝学研究所ホームページResearch Highlights 和文. TEL 03-6402-6200 FAX 03-3431-3070 E-mail:webmaster rc@rois.ac.jp.

thericejournal.springeropen.com

The Rice Pentatricopeptide Repeat Gene TCD10 is Needed for Chloroplast Development under Cold Stress | Rice | Full Text

http://thericejournal.springeropen.com/articles/10.1186/s12284-016-0134-1

Skip to main content. The Rice Pentatricopeptide Repeat Gene. Is Needed for Chloroplast Development under Cold Stress. The Author(s). 2016. In this paper, we characterized a new rice PPR gene mutant. Tcd10 (thermo-sensitive chlorophyll-deficient mutant 10). Encoding a novel PPR protein, mainly localized to the chloroplasts, with 27 PPR motifs, is responsible for the mutant phenotype. In addition,. Is specific expression in tissues. The disruption of. For chloroplast development under cold stress. Encodes...

dbcls.rois.ac.jp

CRISPRdirect ｜ DBCLS

http://dbcls.rois.ac.jp/en/services/sequencing/crisprdirect

DBCLS Database Center for Life Science. AOE (All Of gene Expression). OntoFinder & OntoFactory. CRISPRdirect is a web server for designing CRISPR/Cas guide RNA with reduced off-target sites. ( http:/ crispr.dbcls.jp/. Bioinformatics. 2015 Apr 1;31(7):1120-3. doi: 10.1093/bioinformatics/btu743. Epub 2014 Nov 20. CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites. Naito Y1, Hino K1, Bono H1, Ui-Tei K2. AOE (All Of gene Expression). OntoFinder & OntoFactory.

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OTHER SITES

crispr-on.org

CRISPR-on Home

Read the Article (open access). This is the homepage of CRISPR-on, an RNA-guided transcriptional activator created in the Jaenisch Lab. AOP Aug 27, 2013 Article (Open Access). This page is maintained by Albert Cheng. And is under-construction. We are working with Addgene to complete the collection of plasmids. Read the Article (Open Access). Select plasmids - Plasmid Selection Guide. Get the plasmids - Addgene Page.

crispr-tf.com

CRISPR-on Home

Read the Article (open access). This is the homepage of CRISPR-on, an RNA-guided transcriptional activator created in the Jaenisch Lab. AOP Aug 27, 2013 Article (Open Access). This page is maintained by Albert Cheng. And is under-construction. We are working with Addgene to complete the collection of plasmids. Read the Article (Open Access). Select plasmids - Plasmid Selection Guide. Get the plasmids - Addgene Page.

crispr-tf.org

CRISPR-on Home

Read the Article (open access). This is the homepage of CRISPR-on, an RNA-guided transcriptional activator created in the Jaenisch Lab. AOP Aug 27, 2013 Article (Open Access). This page is maintained by Albert Cheng. And is under-construction. We are working with Addgene to complete the collection of plasmids. Read the Article (Open Access). Select plasmids - Plasmid Selection Guide. Get the plasmids - Addgene Page.

crispr-tfs.com

CRISPR-on Home

Read the Article (open access). This is the homepage of CRISPR-on, an RNA-guided transcriptional activator created in the Jaenisch Lab. AOP Aug 27, 2013 Article (Open Access). This page is maintained by Albert Cheng. And is under-construction. We are working with Addgene to complete the collection of plasmids. Read the Article (Open Access). Select plasmids - Plasmid Selection Guide. Get the plasmids - Addgene Page.

crispr-tfs.org

CRISPR-on Home

Read the Article (open access). This is the homepage of CRISPR-on, an RNA-guided transcriptional activator created in the Jaenisch Lab. AOP Aug 27, 2013 Article (Open Access). This page is maintained by Albert Cheng. And is under-construction. We are working with Addgene to complete the collection of plasmids. Read the Article (Open Access). Select plasmids - Plasmid Selection Guide. Get the plasmids - Addgene Page.

crispr.dbcls.jp

CRISPRdirect

Mdash; Rational design of CRISPR/Cas target. Or Paste a nucleotide sequence:. Sample sequence atgccgcgcgtcgtgcccgaccagagaagcaagttcgagaacgaggagttttttaggaag ctgagccgcgagtgtgagattaagtacacgggcttcagggaccggccccacgaggaacgc caggcacgcttccagaacgcctgccgcgacggccgctcggaaatcgcttttgtggccaca ggaaccaatctgtctctccagttttttccggccagctggcagggagaacagcgacaaaca cctagccgagagtatgtcgacttagaaagagaagcaggcaaggtatatttgaaggctccc atgattctgaatggagtctgtgttatctggaaaggctggattgatctccaaagactggat ggtatgggctgtctggagtttgatgaggagcgagcccagcaggaggatg...

crispr.i2bc.paris-saclay.fr

CRISPR home page

Cas Genes at TIGR. CRISPR plasmids for academic lab research at Addgene. CRISPR evolution ( Yersinia pestis. CRISPRs found ( *. Number of convincing CRISPR structures (number of genomes with such CRISPR). This web site is the product of an original work by Ibtissem Grissa ( PhD thesis Paris University. And is presently developed by Christine Drevet. A web tool to identify clustered regularly interspaced short palindromic repeats. Nucleic Acids Res. 2007 May 31. General purpose of this site. Itself, in wh...

crispr.med.harvard.edu

sgRNA Scorer 1.0

Church lab CRISPR resources. SgRNA Scorer 1.0. SgRNA Scorer 1.0. We have also made available a pre-computed list of sites derived using CasFinder and scored with sgRNA Scorer for the entire human and mouse exomes for both Cas9 orthologs. This can be found here. Please use a working e-mail address as a link to a file with results from sgRNA scorer will be emailed to you as soon as it's done. The file will be retained for 24 hours. S pyogenes (PAM: NGG). S thermophilus (PAM: NNAGAAW).

crispr.sigmainformatics.com

CRISPR/Cas gRNA Designs

Order Oligos and Peptides. Product and Process Development. Safety and Regulatory Information. New Lab Start-Up Program. Search CRISPR/Cas gRNA designs. Review and Email Order. Ordering and Design Guidelines. 1) Search by Gene Symbol, mRNA RefSeq, or Gene ID. 2) Searches are performed on the highlighted tab only. 3) Search for up to 10 terms at one time. Option 1 : Enter Gene Symbol(s). Option 2 : Upload from template. Option 1 : Enter RefSeqs(s). Option 2 : Upload from template. We recommend screening 4...

crispr.u-psud.fr

Maintenance

Le site est desormais accessible a l'adresse suivante :. Http:/ crispr.i2bc.paris-saclay.fr.

crispr.us

crispr.us - This website is for sale! - crispr Resources and Information.

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