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英泰仪器 - 做一流实验室、医用、检测、制药等行业离心机厂家
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1 取肾 用无菌操作取出断乳前后幼龄动物的肾脏置灭菌平皿中，剪去留的被膜和脂肪，并纵向切成两半，剪去肾的被膜和脂肪，并纵向切成两半，剪去肾盂及髓质部分，用含双抗(指青、链霉素，下同)的汉克氏液洗净，移置小烧杯中，剪成乳糜状，再用汉克氏液洗2—3次，至液体澄清无血细胞为止。 2 消化 加入10倍的0.125 胰酶液进行消化。 3 细胞计数和分装 取已制好的细胞悬浮液，用计数白细胞的方法计数，根据计数结果，用营养液(pH6.8—7.0)稀释为每毫升含50-60万细胞，分装于小扁瓶中 容量约50毫升的扁瓶加悬液约5毫升 ，也可分装在链霉素小瓶中。 4 培养 在37 培养，3—4天即可粘附瓶壁形成单层细胞，以后每过3—4大更换一次维持液。 5 接毒 将病料剪碎，按1 4加含双抗的汉克氏液研磨成乳糜状，经冻融处理使细胞破碎，病毒充分释放，用台式离心机，将转速控制在3000转/分左右离心15分钟，将上清液接种到已生长单层细胞的培养瓶中，在37 温箱中吸附30-60分钟，然后加入维持液继续培养。 1将“POWER”打开至“ON”。 如需要紧急刹车 遇故障时 可打开至“ON”档，但一般尽量不用。 12)006 N-苯甲...
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